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عدد المساهمات : 1048
تاريخ التسجيل : 07/03/2011
 | | موضوع: Blood culture الثلاثاء مايو 03, 2011 4:25 am | |
| Blood culture 
[b]When the test is order ?
Blood cultures are collected from patients with suspected sepsis or bacteremia.
Virtually any organism may cause bacteremia. Thus, the isolation of all
organisms from a blood culture must be considered significant and
correlated with the clinical picture.
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what is the sample type?
blood , sterial fluide , CSF , Bone Marrow
طبعاً العينة هنا سواء كانت دم أو نخاع العظم ... إلخ توضع في القوارير Vial التالية :
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How it's work?
The sample to be tested is inoculated into one or more vials which are
inserted into the BACTEC fluorescent series instrument for incubation
and periodic reading. Each vial contains a chemical sensor which can
detect increases in CO2 produced by the growth of microorganisms. The
sensor is monitored by the instrument every ten minutes for an increase
in its fluorescence, which is proportional to the amount of CO2 present.
A positive reading indicates the presumptive presence of viable
microorganisms in the vial. Detection is limited to microorganisms that
will grow in a particular type of medium.
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what is the component of the media in the vials ?
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Blood Culture Systems
Routine Blood Culture BacT/Alert blood culture bottles.
A blood culture set is - One aerobe bottle (adequate for most yeast also) and one anaerobe bottle.
Blood culture bottle set (aerobic and anaerobic)
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Blood culture, neonate
Neonate Blood Bottle (PEDS Plus) for aerobic organisms. (A separate adult anaerobic may be sent if clinically indicated.)
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Incubate Blood culture bottles in the BacT/Alert System
بعد مرحلة السحب و دخول الدم في الـ vials تدخل في هذا الجهاز ..
In the laboratory, blood culture bottles are placed in a blood culture
machine set at 370C. Here the barcode is “read” in order to enter
details of the bottle into the computer of the blood culture machine.
After 12-48 hours metabolic products from a growing population of
bacteria trigger a sensor, and the machine alerts that bottle as being
“positive”.
(يوجد ملف AUTOMATED BLOOD CULTURE BACTEC آخر الموضوع حول عمل الجهاز بجميع التفاصيل )
فترة الـ incubation تختلف باختلاف العينات
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Interpretation of BacT/Alert Blood Culture Bottles
The BacT/Alert System will continuously rock the blood culture bottles
at 70 cycles per minute and scan all bottles (every 10 minutes) for
evidence of growth. The machine will automatically flag any positive
blood cultures.
Negative Cultures
i) Routine, Bone marrow (for sterility testing), sterile fluids, and general fungus/yeast/candida/cryptococcus blood cultures:
Discard all negative bottles after 5 days incubation and issue a negative report.
ii) Bone bank blood cultures:
Discard all negative bottles after 7 days incubation and issue a negative report.
iii) SBE/IE and PUO/FUO, Brucella:
After 21 days incubation, discard all negative bottles and issue a negative report.
Positive Cultures
When the bottle has been flagged positive by the BacT/Alert or is
macroscopically positive (i.e. bulging septum, obvious discoloration or
lemon-yellow sensor disc) process ONLY the suspected positive bottle (do
not sub-culture the matching bottle unless it is also macroscopically
positive or flagged positive). If only one bottle has been flagged
positive, leave the companion bottle in the BacT/Alert until completion
of its incubation time, then discard if negative. Process all suspected
positive bottles as follows:
i) Gram stain
ii) Sub-culture onto the following (whole) plates*:
Media Incubation
Blood Agar (BA) CO2 35°C x 48 hours
Chocolate Agar (CHOC) CO2 35°C x 48 hours
MacConkey Agar (MAC) CO2 35°C x 48 hours
Fastidious Anaerobic Agar (BRUC) AnO2 35°C x 48 hours
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ملفات تحتوي على كامل التفاصيل إن أردت الاستزادة ...
AUTOMATED BLOOD CULTURE BACTEC™.zip
[ندعوك للتسجيل في المنتدى أو التعريف بنفسك لمعاينة هذا الرابط]
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blood culture manule.zip
[ندعوك للتسجيل في المنتدى أو التعريف بنفسك لمعاينة هذا الرابط]
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Blood Culture Collection.zip
[ندعوك للتسجيل في المنتدى أو التعريف بنفسك لمعاينة هذا الرابط]
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